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鈴木, 宏志; 川瀬, 洋介. |
Cryopreservation of mouse spermatozoa has been widely applied for maintenance of genetically modified mouse strains. Although cryopreservation of mouse spermatozoa is simpler, less time-consuming, and less costly than of embryos for preservation of the genetic materials, maintenance of cryopreserved spermatozoa still has a high running cost because of the need for a constant supply of liquid nitrogen. In 1998, it has been reported that freeze-dried mouse spermatozoa are capable of participating in normal embryonic development after injection into oocytes, and so they have attracted a great deal of attention as storable gene resources. However, attempts of freeze-dry spermatozoa are not new. Successful conception and full-term development using freeze-dried... |
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Palavras-chave: Mice; Freese-drying; Spermatozoa; マウス; 凍結乾燥; 精子. |
Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4510 |
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